In situ molecular level studies on membrane related peptides and proteins in real time using sum frequency generation vibrational spectroscopy |
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Authors: | Shuji Ye, Khoi Tan Nguyen, St phanie V. Le Clair,Zhan Chen |
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Affiliation: | aDepartment of Chemistry, University of Michigan, 930 North University Ave., Ann Arbor, MI 48109, USA |
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Abstract: | Sum frequency generation (SFG) vibrational spectroscopy has been demonstrated to be a powerful technique to study the molecular structures of surfaces and interfaces in different chemical environments. This review summarizes recent SFG studies on hybrid bilayer membranes and substrate-supported lipid monolayers and bilayers, the interaction between peptides/proteins and lipid monolayers/bilayers, and bilayer perturbation induced by peptides/proteins. To demonstrate the ability of SFG to determine the orientations of various secondary structures, studies on the interactions between different peptides/proteins (melittin, G proteins, alamethicin, and tachyplesin I) and lipid bilayers are discussed. Molecular level details revealed by SFG in these studies show that SFG can provide a unique understanding on the interactions between a lipid monolayer/bilayer and peptides/proteins in real time, in situ and without any exogenous labeling. |
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Keywords: | Sum frequency generation SFG Cell membrane Antimicrobial peptide Membrane protein Lipid bilayer Melittin G proteins Alamethicin Tachyplesin I |
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