The Feulgen Reaction after Glutaraldehyde Fixation |
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| Authors: | Frederick H. Kasten Raymond Lala |
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| Affiliation: | a Department of Anatomy, Louisiana State University Medical Center, New Orleans, Louisiana |
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| Abstract: | A technique is described for performing the Feulgen reaction for DNA on cells and tissues fixed in glutaraldehyde. Blockade free aldehydes by reducing them with fresh 0.5% NaBH4 in 1% NaH2PO4 for 1 hr at room temperature, then rinse in water. Follow by a Feulgen reaction (hydrolysis at room temperature in 6 N HCI for 20 min, Schiff's reagent for 60 min.). Controls assure the completeness and irreversibility of the borohydride blockade. Cytophotometry shows that the DNA content per nucleus is unaffected by the blockade procedure. |
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