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Human leukocyte interferon inhibits human chorionic gonadotropin stimulated testosterone production by porcine Leydig cells in culture |
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| Authors: | M Orava K Cantell R Vihko |
| Institution: | 1. Department of Clinical Chemistry, University of Oulu, SF-90220 Oulu, Finland;2. National Public Health Institute, SF-00280 Helsinki, Finland |
| Abstract: | Pretreatment of primary porcine Leydig cell cultures with human leukocyte interferon suppressed the subsequent hCG-stimulated testosterone production in a dose-dependent manner, with an ED50 at 13 IU/ml. The treatment had no effect on hCG-binding to its receptor, and the inhibition of testosterone production was not abolished by 8Br-cAMP addition. The results indicate that the site of interferon action on hCG-stimulated testosterone production in primary cultures of porcine Leydig cells is located distal to cAMP formation. |
| Keywords: | IFN interferon P-IFB+A partially purified from human leukocyte interferon NK2-IFN the previous preparation purified with NK2 monoclonal antibody T testosterone Hepes N-2-hydroxyethylpiperazine- N′-2-ethanesulfonic acid PBS phosphate-buffered saline |
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