| 鸡蛋胚下表层卵黄DNA的提取方法 |
| |
| 引用本文： | 孙立军,许怀庆,陈楚楚. 鸡蛋胚下表层卵黄DNA的提取方法[J]. 生物化学与生物物理进展, 1997, 24(1): 82-85 |
| |
| 作者姓名： | 孙立军 许怀庆 陈楚楚 |
| |
| 作者单位： | 中国科学院生物物理研究所, 北京 100101;中国科学院生物物理研究所, 北京 100101;中国科学院生物物理研究所, 北京 100101 |
| |
| 摘 要： | 利用Ficoll-400不连续密度梯度离心将受精和未受精鸡蛋的胚下表层卵黄进行纯化, 显微镜观察表明卵黄球形态良好, 没有胚细胞的存在.然后利用较高浓度的蛋白酶K消化，较长时间的酚抽提，最后提取了DNA. 电泳显示DNA条带清晰.该方法简便快速，从每个鸡蛋的胚下表层卵黄可回收10 ng DNA.
|
| 关 键 词： | DNA提取，卵黄球，密度梯度离心 |
| 收稿时间： | 1996-03-20 |
| 修稿时间： | 1996-08-19 |
Isolation of Yolk DNA from Fertilized and Unfertilized Chichen Eggs |
| |
| SUN Lijun,XU Huaiqing and CHEN Chuchu. Isolation of Yolk DNA from Fertilized and Unfertilized Chichen Eggs[J]. Progress In Biochemistry and Biophysics, 1997, 24(1): 82-85 |
| |
| Authors: | SUN Lijun XU Huaiqing CHEN Chuchu |
| |
| Affiliation: | Institute of Biophysics, Academia Sinica, Beijing 100101, China;Institute of Biophysics, Academia Sinica, Beijing 100101, China;Institute of Biophysics, Academia Sinica, Beijing 100101, China |
| |
| Abstract: | Ficoll-400 discontinuous density gradient centrifugation was used to purify superficial yolk spheres under blastoderms of fertilized chicken eggs and the corresponding site of unfertilized eggs. Under microscope, the purified yolk spheres showed good morphology and no contamination from embronic cells was observed. The purified yolk spheres were lysed and digested with high concentration of proteinase K then extracted for long time with phenol. When subjected to agarose gel electrophoresis, the DNA showed a clear band. The recovery of DNA is approximately 10 ng per fertilized or unfertilized egg. |
| |
| Keywords: | DNA isolation yolk sphere density gradient centrifugation |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
|
点击此处可从《生物化学与生物物理进展》下载全文 |
|
