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Inhibition of Human UDP-Glucose Dehydrogenase Expression Using siRNA Expression Vector in Breast Cancer Cells |
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| Authors: | Jae-Wan?Huh Myung-Min?Choi Seung-Ju?Yang Seung?Yong?Yoon Soo?Young?Choi |
| Institution: | (1) Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, 388-1 Poongnap-dong, 138-736 Seoul, Songpa-gu, Korea;(2) Department of Anatomy and Cell Biology, University of Ulsan College of Medicine, 388-1 Poongnap-dong, 138-736 Seoul, Songpa-gu, Korea;(3) Department of Genetic Engineering, Division of Life Sciences, Hallym University, 200-702 Chunchon, Korea |
| Abstract: | UDP-glucose dehydrogenase (UGDH) catalyzes two oxidations of UDP-glucose to yield UDP-glucuronic acid. Pathological over-production of extracellular matrix components may be linked to the availability of UDP-glucuronic acid, therefore UGDH is a potential therapeutic target. RNA interference (RNAi) has been adapted to knock down the expression of human UGDH. A UGDH siRNA plasmid was constructed using a pRNA-U6.1/Neo vector and transfected into breast cancer cells, ZR-75-1, with an efficiency of up to 50%. Western blot analysis showed that the UGDH expression was efficiently knocked down at protein levels by RNAi in ZR-75-1 cells. |
| Keywords: | breast cancer cell gene knock-down RNA interference siRNA expression vector UDP-glucose dehydrogenase |
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