ESR Spin Trapping Detection of Hydroxyl Radicals in the Reactions of Cr(V) Complexes with Hydrogen Peroxide |
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| Authors: | Xianglin Shi N S Dalal |
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| Institution: |
a Department of Chemistry, West Virginia University, Morgantown, WV, USA
b Department of Biochemistry, University of California at Berkeley, Berkeley, CA |
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| Abstract: | Electron spin resonance (ESR) measurments provide direct evidence for the involvement of Cr(V) in the reduction of Cr(VI) by NAD(P)H. Addition of hydrogen peroxide (H2O2) to NAD(P)H-Cr(VI) reaction mixtures suppresses the Cr(V) signal and generates hydroxyl (OH) radicals (as detected via spin trapping), suggesting that Cr(V) reacts with H2O2 to generate the OH radicals. Reaction between H2O2 and a Cr(V)-glutathione complex. and between H2O2 and several Cr(V)-cdrboxylato complexes also produces OH radicals. These results suggest that Cr(V) complexes catalyze the generation of OH radicals from H2O2, and that OH radicals might play a significant role in the mechanism of Cr(VI) cytotoxicity. |
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| Keywords: | ESR spin trapping Cr(VI) reduction Cr(V) hydrogen peroxide free radicals |
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