Neither total culture fluorescence nor intracellular fluorescence are indicative of NAD(P)H levels in Escherichia coli MG 1655 |
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Authors: | Thomas Hottiger James E Bailey |
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Institution: | (1) Department of Chemical Engineering 210-41, California Institute of Technology, 91125 Pasadena, CA, USA;(2) Present address: Biotechnology Department, CIBA-GEIGY AG, CH-4002 Basel, Switzerland |
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Abstract: | Summary The blue fluorescence emitted by microbial cells irradiated with UV light at 360 nm is usually supposed to provide a good estimate of the cell NAD(P)H content. Here we present an example of a microbial fermentation in which culture fluorescence, both in the cells and in the medium, was almost exclusively due to the presence of a fluorophore that displayed an emission spectrum very similar to that of NAD(P)H but that we show by biochemical studies to be a different compound. Our results demonstrate that studies on the redox state of cells should be based on on-line fluorescence data only after appropriate control experiments to establish a definitive correlation between fluorescence and NAD(P)H levels.
Offprint requests to: J. E. Bailey |
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