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Modified hydroxyalkyl methacrylate gel as a support for immobilization of yeast cells |
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| Authors: | V. Jirků J. Turková A. Kuchynková V. Krumphanzl |
| Affiliation: | (1) Department of Fermentation Chemistry and Technology, Institute of Chemical Technology, 166 28 Prague, Czechoslovakia;(2) Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague, Czechoslovakia;(3) Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague, Czechoslovakia |
| Abstract: | Summary Whole cells of Zygosaccharomyces lactis have been covalently linked to fine-grained hydroxyalkyl methacrylate gel Spheron P 1000 E which was prepared by treatment with epichlorhydrin and modified by an amine spacer. Experiments on the coupling of permeabilized and non-permeabilized cells to this gel support have shown that immobilized cell agregates may be obtained by the immobilization of thermally permeabilized cells. Cell clustering can be bypassed by immobilizing non-permeabilized cells. This immobilization procedure makes additional permeabilization possible. |
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