Effect of the pH of culture medium on the alkalophilicity of a species of Bacillus |
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| Authors: | Noriyuki Koyama Yoshiaki Nosoh |
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| Affiliation: | (1) Laboratory of Chemistry of Natural Products, Tokyo Institute of Technology, 152 Meguroku, Tokyo, Japan |
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| Abstract: | The amino acid incorporation and  -aminoisobutyric acid (AIB) uptake of an alkalophilic Bacillus grown at pH 8.2 (the pH 8-bacteria) were much less pH dependent (less alkalophilic) than those of the organisms grown at pH 10.0 (the pH 10-bacteria), respectively. The rate of AIB uptake of the pH 10-bacteria was almost the same as that of the pH 8-bacteria, while the rate of amino acid incorporation of the pH 10-bacteria was higher than that of the pH 8-bacteria in alkaline environments. The colloidal titration with clupein showed that the amount of negative charge on the pH 10-bacteria was greater than that of the pH 8-bacteria in alkaline environments. Considerable difference in protein composition was observed between the membranes of the pH 8-and 10-bacteria, while no difference was observed in phospholipid composition.Abbreviations AIB Amino-isobutyric acid |
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| Keywords: | Alkalophilic Bacillus Alkalophilicity Loss of alkalophilicity Membrane proteins pH of culture medium |
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