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Monoallelic expression of mouse <Emphasis Type="Italic">Cd4</Emphasis> gene
Authors:Rosanna?Capparelli  Adele?Costabile  Maurizio?Viscardi  Email author" target="_blank">Domenico?IannelliEmail author
Institution:(1) School of Biotechnological Sciences, University of Naples ldquoFederico IIrdquo, Naples, Italy;(2) Chair of Immunology, University of Naples ldquoFederico IIrdquo, Via Università 133, Portici, Naples, 80055, Italy
Abstract:A 7-bp deletion in the Cd4 gene, present in the strain MOLF/Ei of Mus musculus molossinus and absent in laboratory mouse strains (Mus musculus musculus), provided the means to distinguish the parental origin of the Cd4 alleles expressed in single cells of F1 (AKR × MOLF/Ei) and F1 (Balb/C × MOLF/Ei) hybrids. Single-cell RT-PCR showed that the individual CD4+ lymphocyte expresses either the maternal or the paternal Cd4 allele, never both. In situ hybridization proved that Cd4 alleles replicate asynchronously, as expected in the case of genes expressed monoallelically.
Keywords:
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