Development of genetic markers in celery based on restriction fragment length polymorphisms |
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| Authors: | G M Huestis J M McGrath C F Quiros |
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| Institution: | (1) Department of Vegetable Crops, University of California, 95616-8746 Davis, CA, USA;(2) Present address: Department of Biology, University of Michigan, 48109-1048 Ann Arbor, MI, USA |
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| Abstract: | Summary Linkage relationships are reported for 34 markers in celery (Apium graveolens L. var  dulce ) including 21 RFLP, 11 isozyme, and 2 morphological traits. The mapping was carried out in a cross between celery and an annual accession from Thailand, A143, and based on F2 segregation of 136 plants. A total of 318 centiMorgans (cM) are covered by the markers distributed in 8 linkage groups. Probes for the identification of RFLPs were isolated from a celery cDNA library and were also obtained from heterologous sources. EcoRV, EcoRI, and HindIII were the most useful restriction enzymes in uncovering polymorphism. In our cross, 18% of the cDNA probes were found to be polymorphic for at least one of the enzymes used. Six of the markers showed significant deviations from expected F2 ratios. |
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| Keywords: | RFLP mapping Isozymes Apium graveolens |
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