家蚕Bm Tpi基因Z染色体定位

采用实时定量PCR技术，以家蚕Bombyx mori第11号染色体上的DH-PBAN基因为参照基因，检测家蚕不同个体间Bm Tpi基因与常染色体上DH-PBAN基因的拷贝数之比，雄体Bm Tpi∶DH-PBAN=1.0，雌体Bm Tpi∶DH-PBAN=0.5；并用已经定位于Z染色体上的Bm Kettin基因为参照，检测Bm Tpi基因的拷贝数与Bm Kettin基因的拷贝数之比，雄体Bm Tpi∶Bm Kettin=1.0，雌体Bm Tpi∶Bm Kettin=1.0，证明Bm Tpi基因在家蚕基因组中的拷贝数与Bm Kettin基因相同，说明Bm Tpi基因位于Z染色体上。

Localization of the Tpi gene on Z chromosome of the silkworm, Bombyx mori by real-time quantitative PCR

The ratio of copy number between Bm Tpi and DH-PBAN, which is located on the 11th chromosome was detected with the real-time quantitative PCR technique. It was 1.0 in males and 0.5 in females. The ratio of copy number between Bm Tpi and Bm Kettin, which is located on Z chromosome, was same in males and females. This indicated that the copy number of Bm Tpi was equal to that of Bm Kettin in the silkworm genome, i.e. one copy in females and two in males. The results suggest that Bm Tpi is located on Z chromosome in the genome of silkworm.