Growth of Venezuelan Equine Encephalomyelitis Virus in Human Diploid Cell Strain WI-38 |
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Authors: | Morton Reitman and Leonard Green |
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Abstract: | We demonstrated that Venezuelan equine encephalomyelitis (VEE) virus replicated in and adapted rapidly to human diploid cell strain WI-38. Peak titers of approximately 10(9.8) mouse intracerebral 50% lethal doses were obtained at low passage levels in Eagles basal medium supplemented with calf serum. VEE virus replicated poorly in serum-free medium. Propagation of VEE virus was accompanied by the production of hemagglutinin and cytopathogenic effects. |
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