Microsatellite primers for the narrowly endemic shrub Eriogonum giganteum (Polygonaceae) |
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Authors: | Riley Lynn McGlaughlin Mitchell E Helenurm Kaius |
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Affiliation: | Department of Biology, University of South Dakota, 414 East Clark Street, Vermillion, South Dakota 57069 USA. |
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Abstract: | ? Premise of the study: Microsatellite primers were designed for Eriogonum giganteum var. formosum, an endemic shrub of San Clemente Island, to investigate population structure, genetic diversity, and demographic history. ? Methods and Results: Twelve polymorphic microsatellite loci were isolated from the California Channel Island endemic Eriogonum and were screened for variability. The primers amplified one to eight alleles in the target taxon. Many primers also amplified in conspecific and congeneric (E. arborescens, E. fasciculatum, E. grande, E. latifolium, and E. parvifolium) taxa and in the closely related Chorizanthe valida. The total number of alleles per locus for all taxa screened ranged from three to 24. ? Conclusions: These primers will be useful for conservation genetic and evolutionary studies within the California Channel Island endemic Eriogonum. |
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