酪酸梭菌与婴双歧杆菌对艰难梭菌体外生物拮抗作用的作用 |
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引用本文: | 吕存女,邝欣,张雪平,邹开勇,孟筱琦.酪酸梭菌与婴双歧杆菌对艰难梭菌体外生物拮抗作用的作用[J].中国微生态学杂志,2001,13(4):222-224. |
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作者姓名: | 吕存女 邝欣 张雪平 邹开勇 孟筱琦 |
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作者单位: | 兰州生物制品研究所 |
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摘 要: | 用酪酸梭菌(Clostridium butyricum)和婴双歧杆菌(Bifidobacterium infantis)单独或联合艰难菌(Clostridium difficile)进行试管内的生物拮抗作用,将酪酸梭菌、婴儿双歧杆菌单独或酪酸梭菌和婴儿双歧杆菌联合分别与艰难梭菌以一定的比例等量混合后,接种于GAM液体培养基中进行厌氧培养。证明酪酸梭菌和婴儿双歧杆菌能明显抑制艰难梭菌的生长,并且两菌联合比各自单独培养时显示出更强的生物拮抗作用。
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关 键 词: | 酪酸梭菌 婴双歧杆菌 艰难梭菌 拮抗作用 |
文章编号: | 1005-376(2001)04-0222-03 |
修稿时间: | 2000年10月16 |
ANTAGONISM OF CLOSTRIDIUM BUTYRICUM AND BIFIDOBACTERIUM INFANTIS TO CLOSTRIDIUM DIFFICILE IN VITRO |
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Abstract: | This paper investigated antagonism of Bifidobacterium infantis LCL172 and CLostridium butyricum LCL166 against CLostridium difficile VPI10463 strain in vitro Either of LCL166 and LCL172 or both was mixed with proportionally C difficile in GAM culture media respectively In the meantime,C difficile was incubated in GAM culture as a control During anaerobic incubation C difficile was counted at intervals As a result the multiplication of C difficile was inhibited by either of LCL172 or LCL166 or both of them significantly Both were able to restrain more multiplication of C difficile than either of them It may be resulted from the inhibiting action of the organic acid produced by LCL172 and LCL166 by which lowed the growth of C difficile was inhibited due to the decreased pH of the culture |
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Keywords: | Clostridium butyricum Bifidobacterium infantis Clostridium difficile Antagonism |
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