Effects of protein deficiency on selective elicitation of lysosomal enzymes in rat peritoneal macrophages |
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Authors: | U R Iyengar U K Vakil |
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Institution: | (1) Biochemistry and Food Technology Division, Bhabha Atomic Research Centre, Trombay, 400 085 Bombay, India |
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Abstract: | Young albino rats were fedad libitum 4, 8 or 20 % (control) protein diet for 1–4 weeks. Total activities of some of the lysosomal enzymes, namely, acid phosphatase,
aryl sulphatase, Β-glucuronidase and cathepsin D, were determined in resident and protease-peptone elicited peritoneal macrophages.
Total cell number, protein content and the lysosomal enzyme activities were increased significantly in protease-peptone elicited
macrophages; though at a lower rate in 4 % protein-fed group compared to control ones. However, the rate of induction of the
tested hydrolases was selective and their response to the stimulant varied widely. Similarly, response of each enzyme to low
protein diet also varied. Thus, at 4 weeks, cathepsin D and Β-glucuronidase activities, expressed per total number of elicited
macrophages were reduced by 45 and 60 %, respectively, in 4 % protein-fed animals. These results indicate that the metabolic
events related to lysosomal function in macrophages, are affected by dietary restriction of proteins |
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Keywords: | Rat protein deficiency peritoneal macrophages elicitation lysosomal enzymes |
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