Production of transgenic maize plants and progeny by bombardment of hi-II immature embryos |
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Authors: | D. D. Songstad C. L. Armstrong W. L. Petersen B. Hairston M. A. W. Hinchee |
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Affiliation: | (1) Monsanto, 700 Chesterfield Parkway North, 63198 St. Louis, Missouri;(2) Pioneer Hi-Bred International, Inc., 7300 N.W. 62nd Avenue, P.O. Box 1004, 50131-1004 Johnston, Iowa |
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Abstract: | Summary Production of transgenic maize (Zea mays L.) callus, plants, and progeny from microprojectile bombardment of 2–5-d cultured Hi-II immature embryos is described. Histological evidence indicates that these tissues are amenable to transformation due to surface layer cell division of the scutellum. Two out of every 100 bombarded embryos produced transgenic callus and R0 transgenic plants were both male and female fertile. Expected segregation of transgenes was observed in progeny. The primary advantage of bombarding these tissues is increased male and female fertility of transgenic plants compared with those produced using long-term callus or suspension cultures. |
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Keywords: | transformation Zea mays L histology progeny |
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