Response of stem explants to screening and explant source as a basis for methodical advancing of regeneration protocols for chrysanthemum |
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Authors: | Annadana Seetharam Rademaker Wim Ramanna M Udayakumar M Jong Jan de |
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Institution: | (1) Dipartimento di Coltivazione e Difesa delle Specie Legnose, Sezione Coltivazioni Arboree, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy;(2) Scuola di Studi Universitari e Perfezionamento ‘S.Anna’, Via Carducci 40, 56100 Pisa, Italy |
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Abstract: | The effects of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration, length of induction period and light quality on leaf
regeneration of quince clone BA 29 were investigated. After 2, 4 or 6 days of induction with 2.5 mg l−1 or 5.0 mg l−1 2,4-D, leaves were cultured under red, blue, red+blue, far-red+blue, white, far-red light or darkness conditions. Leaves
thereby treated showed different responses, with respect to somatic embryogenesis, callus, red-nodular structures or roots.
Callus production increased with increasing 2,4-D concentration and induction period, although it was not influenced by light
quality; the only exception was far-red+blue light, which reduced callusing response. This result suggested involvement of
the blue-absorbing photoreceptor system in the callus formation processes. A high regeneration of red-nodular structures with
a meristematic appearance was also observed; from some histological characterizations, we presumed they were adventitious
buds that were arrested at an early developmental stage. Red-nodular structures increased with decreasing 2,4-D concentration
and induction period. In the regeneration of such structures, the blue-absorbing photoreceptor system appeared to have a negative
effect but only at a low photoequilibrium value. In contrast, light quality which activated phytochrome induced an increment
in regeneration, but the response did not vary for photoequilibrium values ranging from 0.43 to 0.86. For root regeneration,
phytochrome seemed to be the only photoreceptor involved.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Cydonia oblonga photoreceptors |
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