<Emphasis Type="Italic">Arabidopsis</Emphasis> RETINOBLASTOMA-RELATED PROTEIN 1 is involved in G1 phase cell cycle arrest caused by sucrose starvation |
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Authors: | Hiroto Hirano Hirofumi Harashima Atsuhiko Shinmyo Masami Sekine |
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Institution: | (1) Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama 8916-5, Ikoma Nara, 630-0101, Japan;(2) Department of Bioproduction Science, Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Suematsu 1-308, Nonoichimachi Ishikawa, 921-8836, Japan |
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Abstract: | Although sucrose availability is crucial for commitment to plant cell division during G1 phase by controlling the expression
of D-type cyclins, it has remained unclear how these factors mediate entry into the cell cycle. Here we show that Arabidopsis RETINOBLASTOMA-RELATED PROTEIN 1 (AtRBR1) is involved in G1-phase cell cycle arrest caused by sucrose starvation. We generated
estrogen-inducible AtRBR1 RNA interference (RNAi) Arabidopsis suspension MM2d cells, and found that downregulation of AtRBR1 leads to a higher frequency of arrest in G2 phase, instead
of G1-phase arrest in the uninduced control, after sucrose starvation. Synchronization experiments confirmed that downregulation
of AtRBR1 leads to a prolonged G2 phase and delayed activation of G2/M marker genes. Downregulation of AtRBR1 also stimulated
the activation of E2F-regulated genes when these genes were repressed in the uninduced cells under the limited sucrose conditions.
We conclude that AtRBR1 is a key effector for the ability of sucrose to modulate progression from G1 phase. |
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Keywords: | Arabidopsis MM2d cells RB Sucrose starvation |
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