Analysis of coenzyme m (2-mercaptoethanesulfonic acid) derivatives by isotachophoresis |
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| Authors: | J M Hermans T J Hutten C van der Drift G D Vogels |
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| Affiliation: | Department of Microbiology, Faculty of Science, University of Nijmegen, Toernooiveld, 6525 ED Nijmegen, The Netherlands |
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| Abstract: | 2-Mercaptoethanesulfonic acid (coenzyme M, HS-CoM), methylcoenzyme M (CH3-S-CoM), acetylcoenzyme M (CH3CO-S-CoM), 2,2′-dithiodiethanesulfonic acid [(S-CoM)2], and bromoethanesulfonic acid can be simultaneously and conveniently determined by isotachophoretic analysis. Amounts as low as 10 pmol can be detected. The reproducibility of the method is within 2%. The reduction of (S-CoM)2 and the formation of CH3-S-CoM from HS-CoM and methanol by dialyzed cell-free extracts of Methanosarcina barkeri were studied. |
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