锌对急性缺氧小鼠海马NOS和nNOS水平的影响

目的：观察锌对急性缺氧小鼠海马一氧化氮合酶（nitric oxide synthase,NOS）和神经元型一氧化氮合酶（neuronal NOS,nNOS）阳性神经元的影响,以探讨锌抗脑缺氧的作用机制。方法：复制小鼠急性缺氧模型,采用NADPH-d组织化学和nNOS免疫组织化学方法,研究给锌组和不给锌组急性缺氧小鼠海马各分区NOS和nNOS阳性神经元数量的变化。结果：给锌组比不给锌组小鼠缺氧耐受时间显著延长,差异有显著性（P〈0．05）;海马及其CA1区NOS和nNOS阳性神经元的数量明显减少,差异有显著性（P〈0．05）。结论：急性缺氧时锌通过减少海马NoS和nNOS水平而发挥其抗脑缺氧作用。

Effects of zinc on activity of NOS and expression of nNOS in hippocampus of acute hypoxic mice

Aim: To explore the effect of zinc on vigor of nitric oxide synthase(NOS) and the expression level of neuronal nitric oxide synthase(nNOS) in the hippocampus of acute hypoxic mice and the protective effects of zinc. Methods: Model of acute hypoxic mice was duplicated, NADPH-d histochemistry and nNOS immunohistochemistry were used to investigate the changes of NOS in different groups. Results: Compared with the NS group, the hypoxia endurance of the zinc group was significantly increased;the number of NOS positive neurons and nNOS positive neurons in hippocampus and CA1 were significantly decreased. Conclusion: Zinc might play an important role in decreasing the level of NOS in hippocampus to protect the brain against hypoxic damage.