Activation of in vitro maturing oocytes of the Spanish newt as affected by cycloheximide

The P. waltlii oocytes matured in vitro are activated as a result of cycloheximide (CH) treatment. The female nucleus formation was completed in all activated oocytes within 8 h after the onset of treatment. Activation was induced by 0.5 micrograms/ml CH and 5 micrograms/ml CH induced activation in all treated oocytes.