| 利用SPR原理研究mGAT—1基因5‘区域和组织核蛋白的相互作用 |
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| 引用本文： | 王琼,费俭.利用SPR原理研究mGAT—1基因5‘区域和组织核蛋白的相互作用[J].实验生物学报,1999,32(3):221-225. |
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| 作者姓名： | 王琼 费俭 |
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| 摘 要： |
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| 关 键 词： | mGAT－1 组织核蛋白 转运蛋白 神经递质 基因调控 |
Study on the interaction between the 5' proximal region of mGAT-1 and nuclear proteins by the method of SPR] |
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| Q Wang,G Q Cai,J Fei,M Y Zhou,L H Guo.Study on the interaction between the 5' proximal region of mGAT-1 and nuclear proteins by the method of SPR][J].Acta Biologiae Experimentalis Sinica,1999,32(3):221-225. |
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| Authors: | Q Wang G Q Cai J Fei M Y Zhou L H Guo |
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| Institution: | Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China. |
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| Abstract: | The DNA fragment (named F182) corresponding the position of -1775(-)-1594 in the mouse GABA transporter 1 (mGAT-1) 5' proximal region was amplified by PCR. Then the DNA was immobilized to the surface of sensor chip SA5 via biotin-streptavidin linkage. The interaction between the F182 on SA5 and nuclear proteins from mouse liver and kidney was studied by the method of SPR with Biosensor of BIAcore-1000 respectively. The Binding between F182 and two nuclear proteins was definitely and specifically and both with the apparent dissociation rate of about 1.4E-5/s. Competitive experiment revealed that a conserved sequence within F182 had the main contribution to the binding event. |
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