Retroviral Splicing Suppressor Requires Three Nonconsensus Uridines in a 5′ Splice Site-Like Sequence |
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| Authors: | Robert E Paca Catherine S Hibbert Christopher T O'Sullivan and Karen L Beemon |
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| Institution: | Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA. |
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| Abstract: | Rous sarcoma virus RNA contains a negative regulator of splicing (NRS) element that aids in maintenance of unspliced RNA. The NRS binds U1 snRNA at a sequence that deviates from the 5' splice site consensus by substitution of U's for A's at three positions: -2, +3, and +4. All three of these U's are important for NRS-mediated splicing suppression. Substitution of a single nonconsensus C or G at any of these sites diminished NRS activity, whereas substitution of a single A generated a preferred 5' splice site within the NRS. |
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