Peptidomic analysis of human reflex tear fluid |
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| Institution: | 1. Research Group of Functional Genomics and Proteomics, KU Leuven, B-3000 Leuven, Belgium;2. VITO, Boeretang 200, 2400 MOL, Belgium;3. CFP/Ceproma, University Antwerpen, Groenenborgerlaan 171, 2020 Antwerpen, Belgium;4. Laboratory of Gene Technology, KU Leuven, B-3001 Leuven, Belgium;5. Biomedical Sciences Group, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, B-3000 Leuven, Belgium;1. Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA;2. Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA;3. Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA;1. Department of Pathology and Laboratory Medicine, Dalhousie University, 5788 University Avenue, Halifax, Nova Scotia B3H 2Y9, Canada;2. Departments of Pathology and Laboratory Medicine and Family Medicine, Cumming School of Medicine, University of Calgary, 3535 Research Rd. NW, Calgary, Alberta T2L 2K7, Canada;1. Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland;2. Department of Gerodontology and Oral Pathology, University of Medical Sciences, Poznan, Poland;1. Pondicherry University (A Central University of India), India;2. Periyar Govt. College, Cuddalore, India;3. National University of Kaohsiung, Taiwan;1. QOPNA, Mass spectrometry center, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal;2. School of Health Sciences, University of Aveiro, Portugal;3. Laboratory for Bone Metabolism and Regeneration–Faculty of Dental Medicine, U. Porto, Porto, Portugal;4. MedInUP–Center for Drug Discovery and Innovative Medicines, University of Porto, Porto, Portugal |
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| Abstract: | Tear fluid is a complex mixture of biological compounds, including carbohydrates, lipids, electrolytes, proteins, and peptides. Despite the physiological importance of tear fluid, little is known about the identity of its endogenous peptides. In this study, we analyzed and identified naturally occurring peptide molecules in human reflex tear fluid by means of LC-MALDI-TOF–TOF. Tandem MS analyses revealed 30 peptides, most of which have not been identified before. Twenty-six peptides are derived from the proline-rich protein 4 and 4 peptides are derived from the polymeric immunoglobulin receptor. Based on their structural characteristics, we suggest that the identified tear fluid peptides contribute to the protective environment of the ocular surface. |
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