Stearylated antimicrobial peptide [D]-K6L9 with cell penetrating property for efficient gene transfer |
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| Institution: | 1. The First Clinical Medical School, Lanzhou University, Lanzhou 730000, China;2. Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences; Institute of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China;1. Department of Organic Chemistry, Faculty of Pharmacy, Wrocław Medical University, Borowska street 211A, 50-556, Wrocław, Poland;2. Computer Modeling Centre, DMW Communication, Świętokrzyska street 40b, 50-327, Wrocław, Poland;3. Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okólna 2 street, 50-422, Wrocław 2, Poland;1. INFIQC, CONICET and Departamento de Química Teórica y Computacional, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba 5000, Argentina;2. Department of Chemistry, University of North Florida, 1,UNF Drive, Jacksonville, FL, 32224, USA;1. Institut für Pharmazeutische und Medizinische Chemie der Westfälischen Wilhelms-Universität Münster, Corrensstraße 48, D-48149 Münster, Germany;2. Research Center for Materials Science and Department of Chemistry, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan;3. Cells-in-Motion Cluster of Excellence (EXC 1003—CiM), University Münster, Germany;1. Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721, USA;2. Department of Pharmacology, University of Arizona, Tucson, AZ 85719, USA;1. Sandor Life Sciences Pvt Ltd, Banjara Hills, Road No. 3, Hyderabad 500034, India;2. School of Chemical & Biotechnology, SASTRA University, Tirumalaisamudram, Thanjavur 613401, India;3. Center of Excellence in Biotechnology Research & College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia;4. Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia;5. Department of Clinical Pharmacology and Therapeutics, Nizam''s Institute of Medical Sciences, Hyderabad 500082, India;1. MOSE-DEA, University of Trieste, Piazzale Europa 1, 34127 Trieste, Italy;2. Institut für Pharmazeutische und Medizinische Chemie der Westfälischen Wilhelms-Universität Münster, Corrensstraße 48, D-48149 Münster, Germany;3. Institut für Pharmazeutische Biologie und Phytochemie der Westfälischen Wilhelms-Universität Münster, Corrensstraße 48, D-48149 Münster, Germany;4. Cells-in-Motion Cluster of Excellence (EXC 1003 – CiM), University Münster, Germany |
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| Abstract: | Stearyl-cell penetrating peptides (CPPs) have been proved to be efficient nonviral gene vectors. Due to the similarities between antimicrobial peptides and CPPs, we constructed a novel type of gene vectors by introducing stearyl moiety to the N-terminus of antimicrobial peptide D]-K6L9. In this study, stearyl-D]-K6L9 delivered plasmids into cells by clathrin- and caveolin-mediated endocytosis. Gratifyingly, stearyl-D]-K6L9 exhibited high transfection efficiency and almost reached the level of Lipofectamine 2000. Taken together, the combination of the stearyl moiety with D]-K6L9 provides a novel framework for the development of excellent nonviral gene vectors. |
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| Keywords: | Nonviral gene vector Stearylation Cell penetrating peptide Antimicrobial peptide Endocytosis |
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