| 蝴蝶兰EST-SSRs分析 |
| |
| 引用本文： | 张君毅,陈瑞凤.蝴蝶兰EST-SSRs分析[J].植物生理学通讯,2010(6):559-563. |
| |
| 作者姓名： | 张君毅 陈瑞凤 |
| |
| 作者单位： | 华侨大学生物工程与技术系,福建厦门361021 |
| |
| 基金项目： | 福建省农业科技重点项目（2009N0041）; 泉州重点科技项目（2008N40） |
| |
| 摘 要： | 对蝴蝶兰属EST序列进行了SSR分析。蝴蝶兰属EST总长为4.5Mb,含有609个SSR。SSR出现频率7.65%,平均距离7.39kb,平均长度为22.17bp。单碱基、二碱基和三碱基重复是主要重复类型,分别占EST-SSR总数的21.67%、40.39%和33.50%。A、AG和CCG分别是单碱基、二碱基和三碱基重复中主导重复基元,分别占96.21%、58.54%和32.25%。设计引物及检测的结果表明,蝴蝶兰EST-SSR标记对兰科其他属植物具有一定的通用性。
|
| 关 键 词： | 蝴蝶兰 SSR EST 微卫星 |
EST-SSRs Analysis of Moth Orchids |
| |
| ZHANG Jun-Yi,CHEN Rui-Feng.EST-SSRs Analysis of Moth Orchids[J].Plant Physiology Communications,2010(6):559-563. |
| |
| Authors: | ZHANG Jun-Yi CHEN Rui-Feng |
| |
| Institution: | ( Department of Bioengineering ＆ Biotechnology,Huaqiao University,Xiamen,Fujian 361021,China ) |
| |
| Abstract: | Simple sequence repeats （SSRs） were investigated in expressed sequence tags （EST） of moth orchids.The total length of moth orchids non-redundant ESTs was 4.5 Mb,including 609 SSRs.The frequency of ESTSSRs was 7.65% and the mean distance was 7.39 kb.The mean length of SSRs was 22.17 bp.Mononucleotide,dinucleotide and trinucleotide repeats were major types,whicn accounted for 21.67%,40.39% and 33.50% in all SSRs,respectively.A,AG and CCG were mainly frequent motifs in mononucleotide,dinucleotide and trinucleotide,accounting for 96.21%,58.54% and 32.25%,respectively.Primers were designed and detected.The result showed that some of them could be transferable to other genera of orchid. |
| |
| Keywords: | moth orchids SSR EST microsatellite |
| 本文献已被 维普 等数据库收录！ |
|
