| 水稻MYB转录因子OsDUO1的cDNA克隆及其表达模式初步分析 |
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| 引用本文： | 李素玲,宗杰,周志刚,David Twell,张大兵. 水稻MYB转录因子OsDUO1的cDNA克隆及其表达模式初步分析[J]. 植物生理学通讯, 2010, 0(10): 1033-1039 |
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| 作者姓名： | 李素玲 宗杰 周志刚 David Twell 张大兵 |
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| 作者单位： | [1]上海海洋大学水产与生命学院,上海201306 [2]上海交通大学生命科学与技术学院,上海200240 [3]Department of Biology,University of Leicester, United Kingdom LE17RH |
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| 基金项目： | 国家自然科学基金项目（2009CB941500） |
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| 摘 要： | 我们利用RT-PCR方法成功从水稻中克隆了R2R3类MYB转录因子OsDUO1（Oryza sativa duo pollen1）的全长为1032bp的cDNA,该基因编码一个343个氨基酸残基的蛋白。RT-PCR分析结果表明OsDUO1只在水稻的花粉发育后期表达,说明OsDUO1可能对水稻花粉发育具有生物学功能。生物信息学分析表明,OsDUO1在短柄草、高粱、玉米、拟南芥、烟草、葡萄、蓖麻、杨毛果、小立碗藓植物中有相近同源序列,暗示该基因在进化中具有保守的生物学功能。
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| 关 键 词： | 水稻 OsDUO1 克隆 表达模式 进化分析 |
Cloning and Expression Analysis of OsDUO1 Encoding a Rice MYB Transcription Factor |
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| LI Su-Ling,ZONG Jie,ZHOU Zhi-Gang,David Twell,ZHANG Da-Bing. Cloning and Expression Analysis of OsDUO1 Encoding a Rice MYB Transcription Factor[J]. Plant Physiology Communications, 2010, 0(10): 1033-1039 |
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| Authors: | LI Su-Ling ZONG Jie ZHOU Zhi-Gang David Twell ZHANG Da-Bing |
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| Affiliation: | 2,＊ 1College of Fisheries and Life,Shanghai Ocean University,Shanghai 201306,China;2School of Life Science and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China;3Department of Biology,University of Leicester,United Kingdom LE17RH |
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| Abstract: | We successfully cloned the full cDNA sequence of OsDUO1（Oryza sativa duo pollen 1） encoding a putative R2R3 type MYB transcription factor by RT-PCR.The OsDUO1 cDNA is 1 032 bp in length and encodes a protein with 343 amino acids.The expression of OsDUO1 was detectable only during the late pollen development in rice,suggesting the possible biological function of OsDUO1 during late rice pollen development.Bioinformatics analysis showed that OsDUO1 has close homologs in Brachypodium distachyon,Sorghum bicolor,Zea mays,Arabidopsis thaliana,Nicotiana tabacum,Vitis vinifera,Ricinus communis,Populus trichocarpa and Physcomitrella patens suggesting the conserved function of OsDUO1 in plants during evolution. |
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| Keywords: | rice OsDUO1 clone expression pattern phylogenetics analysis |
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