不同激素配比和AgNO3对两种基因型大白菜子叶离体再生的影响

以‘08S555’和‘陕秋白’大白菜子叶为外植体,研究了激素配比和AgNO3对不定芽再生的影响。结果表明：‘陕秋白’的再生率高于‘08S555’,但出芽系数比‘08S555’低。在6-BA与NAA组合时,‘陕秋白’再生率最高,为68.33%,‘08S555’为28.33%;再生系数分别为1.09和2.81。在TDZ与NAA组合时,二者不定芽的再生频率分别为79.17%和45.83%;再生系数分别为1.24和2.55。这说明与6-BA相比,TDZ与NAA组合对两个大白菜子叶再生率的效果更好。AgNO3与细胞分裂素及生长素配合使用,能提高‘陕秋白’子叶不定芽的再生频率。当TDZ浓度为0.3mg·L^-1、NAA为0.5mg·L^-1和AgNO3为6mg·L^-1时,‘陕秋白’再生率最高,达到87.50%。

Effects of Different Hormone Combinations and AgNO_3 on in vitro Regeneration from Cotyledons of Two Genotypes of Heading Chinese Cabbage

Cotyledons of ＇08S555＇ and ＇shanqiubai ＇ were used as explants to study the effects on regeneration of adventitious buds so as to choose optimal hormone combination with AgNO3.The results showed that ＇shanqiubai＇ had higher regeneration and lower sprouting number of explants than ＇08S555＇.In the 6-BA and NAA combinations,＇shanqiubai＇ got the highest regeneration rate of 68.33%,but ＇08S555＇ was only 28.33%.Regeneration coefficients were 1.09 and 2.81.In TDZ and NAA combinations,79.17% and 45.83% of adventitious bud in ＇shanqiubai＇ and ＇08S555＇ regenerated,with regeneration coefficients of 1.24 and 2.55,respectively.With 6-BA in comparision,TDZ was more effective than 6-BA.AgNO3 used in conjunction with the cytokinin and auxin can enhance cotyledon regeneration frequency of ＇shanqiubai＇ adventitious buds.When 6-BA/TDZ combinated with NAA and AgNO3,the highest regeneration rate is 87.50% of ＇shanqiubai＇,and the suitable concentration was TDZ （0.3 mg·L^-1）,NAA （0.5 mg·L^-1） and AgNO3 （6 mg·L^-1） separately.