高山离子芥CbMAPK3基因功能分析

将高山离子芥丝裂原活化蛋白（MAP）激酶基因CbMAPK3编码区连接到表达载体pET-30a中，转化大肠杆菌JJ001（srlzTn10），在1mol·L-1山梨醇的渗透胁迫及IPTG的诱导下，转化pET-30a—CbMAPK3的大肠杆菌JJ001（srl：Tn10）生长情况比转化pET-30a的好。免疫分析表明，在0℃下处理时，高山离子芥CbMAPK3激酶蛋白质水平没有明显的变化；在4℃处理下，高山离子芥CbMAPK3激酶蛋白质水平在处理前3h明显增加，并在3h后达到最高值。高山离子芥CbMAPK3在响应环境胁迫的过程中起作用。

Functional Analysis of CbMAPK3 Gene in Chorispora bungeana Fisch. et Mey.

Chorispora bungeana is a rare alpine subnival plant species that is highly tolerable to freezing environment. To investigate whether mitogen-activated protein （MAP） kinase CbMAPK3 of C. bungeana has the osmo-protection function, the coding region was subcloned into prokaryotic expression vector pET-30a. In the presence of 1 mol.L-1 sorbitol and induction with IPTG, Escherichia coli strain JJ001 （srl：：Tn10） trans-formed with pET-30a-CbMAPK3 exhibited better growth than the same strain transformed with the empty vector. Western blot analysis showed that the protein level of CbMAPK3 was not significantly altered by 0℃ freezing treatment, but increased significantly at 4℃ cold treatment and the maximum level was observed at 3 h after the treatment. These results indicated that the CbMAPK3 might play an role in response to environmental stresses.