Depletion of abundant human serum proteins by per se imprinted cryogels based on sample heterogeneity |
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Authors: | Chun Yang Ya‐Ru Liu Yan Zhang Jian Wang Li‐Li Tian Ya‐Nan Yan Wei‐Qin Cao Yu‐Yang Wang |
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Institution: | 1. School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu Province, P. R., China;2. Testing Centre, Yangzhou University, P. R., China |
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Abstract: | Macroporous cryogels were prepared and used to deplete abundant proteins. It was accomplished based on the sample heterogeneity rather than any exogenous assistance. Human serum was added in monomer solutions to synthesize molecularly imprinted polymers; therein some abundant proteins were imprinted in the polyacrylamide cryogels. Meanwhile the rare components remained aqueous. Chromatography and electrophoresis showed that albumin, serotransferrin, and most globulins were depleted by columns packed with the molecularly imprinted polymers. After the depletion, lower abundance proteins were revealed by SDS‐PAGE, peptide fingerprint analysis, and identified by MALDI‐TOF‐MS. This is an example that a “per se imprint” protocol enables to gradually dimidiate proteomes, simplify sample complexities, and facilitate further proteome profiling or biomarker discovery. |
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Keywords: | Depletion Human serum Molecularly imprinted polymer Protein |
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