Hyperproduction of N-acetylneuraminate lyase by the gene-cloned strain ofEscherichia coli |
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| Authors: | Kazuo Aisaka Shigeaki Tamura Yuko Arai Takayuki Uwajima |
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| Affiliation: | (1) Tokyo Research Laboratory, Kyowa Hakko Kogyo Co., Machida-shi, Tokyo 194, Japan;(2) Fuji Factory, Kyowa Hakko Kogyo Co., Nagaizumi-cho, Shizuoka-ken 411, Japan |
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| Abstract: | Summary Optimal culture medium for production ofN-acetylneuraminate lyase (NANA lyase) by the gene-cloned strain ofEscherichia coli, E. coli(pNALl), was screened to develop the process for the industrial production of NANA lyase. Out of nutrients tested, corn steep liquor (CSL) and sugarcane molasses were superior nutrient sources for the enzyme production. Hyperproduction (6–8 units/ml-broth) was achieved on the medium consisted of CSL and molasses. |
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