Transfer RNA maturation in Chlamydomonas mitochondria,chloroplast and the nucleus by a single RNase P protein |
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Authors: | Mathilde Arrivé Franziska Pinker Thalia Salinas‐Giegé Philippe Giegé |
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Affiliation: | Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, Strasbourg, France |
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Abstract: | The maturation of tRNA precursors involves the 5′ cleavage of leader sequences by an essential endonuclease called RNase P. Beyond the ancestral ribonucleoprotein (RNP) RNase P, a second type of RNase P called PRORP (protein‐only RNase P) evolved in eukaryotes. The current view on the distribution of RNase P in cells is that multiple RNPs, multiple PRORPs or a combination of both, perform specialised RNase P activities in the different compartments where gene expression occurs. Here, we identify a single gene encoding PRORP in the green alga Chlamydomonas reinhardtii while no RNP is found. We show that its product, CrPRORP, is triple‐localised to mitochondria, the chloroplast and the nucleus. Its downregulation results in impaired tRNA biogenesis in both organelles and the nucleus. CrPRORP, as a single‐subunit RNase P for an entire organism, makes up the most compact and versatile RNase P machinery described in either prokaryotes or eukaryotes. |
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Keywords: | RNase P evolution protein‐only RNase P tRNA biogenesis Chlorophyta |
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