KymoRod: a method for automated kinematic analysis of rod‐shaped plant organs |
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Authors: | Renaud Bastien David Legland Marjolaine Martin Lucien Fregosi Alexis Peaucelle Stéphane Douady Bruno Moulia Herman Höfte |
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Institution: | 1. Institut Jean‐Pierre Bourgin, INRA, Centre National pour la Recherche Scientifique, AgroParisTech, Université Paris‐Saclay, Versailles Cedex, France;2. Department of Collective Behaviour, Max Planck Institute for Ornithology and Department of Biology, University of Konstanz, Konstanz, Germany;3. Biopolymères Interaction et Assemblages, INRA, UR1368, Nantes, France;4. Matière et Systèmes Complexes, Université Paris‐Diderot, Paris Cedex 13, France;5. INRA, UMR 547 PIAF, Clermont‐Ferrand, France;6. Clermont Université, Université Blaise Pascal, UMR 547 PIAF, Clermont‐Ferrand, France |
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Abstract: | A major challenge in plant systems biology is the development of robust, predictive multiscale models for organ growth. In this context it is important to bridge the gap between the, rather well‐documented molecular scale and the organ scale by providing quantitative methods to study within‐organ growth patterns. Here, we describe a simple method for the analysis of the evolution of growth patterns within rod‐shaped organs that does not require adding markers at the organ surface. The method allows for the simultaneous analysis of root and hypocotyl growth, provides spatio‐temporal information on curvature, growth anisotropy and relative elemental growth rate and can cope with complex organ movements. We demonstrate the performance of the method by documenting previously unsuspected complex growth patterns within the growing hypocotyl of the model species Arabidopsis thaliana during normal growth, after treatment with a growth‐inhibiting drug or in a mechano‐sensing mutant. The method is freely available as an intuitive and user‐friendly Matlab application called KymoRod. |
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Keywords: | kinematics image analysis hypocotyl root elongation growth mechano‐sensing cellulose synthesis inhibitor
Arabidopsis thaliana
technical advance |
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