Galactose induces the expression of penicillin acylase under control of the lac promoter in recombinant Escherichia coli |
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| Authors: | Antonio De León Graciela B. Breceda Ana P. Barba de la Rosa Juan F. Jiménez-Bremont Rubén López-Revilla |
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| Affiliation: | (1) Departamento de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Apartado Postal 3-74 Tangamanga, 78231 San Luis Potosí, S.L.P., México;(2) Instituto Tecnológico de Celaya, Av. Tecnológico, 38040 Celaya, Gto., México |
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| Abstract: | The expression of penicillin acylase (PA), cloned in the pPA102 plasmid under control of the wild-type lac promoter and using galactose as inducer in Escherichia coli JM101, JM103 and JM105 transformant cells, was analyzed. The E. coli JM101/pPA102 cultures attained the highest specific activity of PA. For large scale PA production based on E. coli JM101/pPA102 a culture media with galactose instead of isopropyl-thio-galactopyranoside as inducer would be as successful and less expensive. |
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| Keywords: | expression galactose induction lac promoter penicillin acylase pPA102 |
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