Effects of tumor promoters,genotoxic carcinogens and hepatocytotoxins on mouse hepatocyte intercellular communication |
| |
Authors: | Randall J Ruch James E Klaunig |
| |
Institution: | (1) Department of Pathology, Medical College of Ohio, Toledo, Ohio;(2) Department of Pathology, Medical College of Ohio, 3000 Arlington Avenue, 43699 Toledo, OH |
| |
Abstract: | Intercellular communication via gap junctions may be an important mechanism of cellular growth control. Tumor promoters can inhibit intercellular communication between cultured cells, while genotoxic carcinogens apparently lack this capability. The inhibition of intercellular communication by tumor promoters may be an essential mechanism by which tumor promotion occurs in vivo. In this study, the liver tumor promoters phenobarbital, lindane (1,2,3,4,5,6-hexachlorocyclohexane, -isomer), DDT (1,1-Bis4-chlorophenyl],-2,2,2-trichloroethane), Aroclor 1254 (a polychlorinated biphenyl mixture) and dieldrin inhibited intercellular communication between male B6C3F1 mouse hepatocytes in primary culture. Intercellular communication was detected as the passage of 5-3H]uridine nucleotides from pre-labelled donor hepatocytes to non-labelled recipient heptocytes. Mouse hepatocyte intercellular communication was also inhibited by the skin tumor promoter TPA (12-0-tetradecanoyl phorbol-13-acetate), but not by the bladder tumor promoter saccharin. The genotoxic hepatocarcinogens dimethylnitrosamine, diethylnitrosamine, benzoa]pyrene and 2-acetylaminofluorene, and the hepatocytotoxins bromobenzene, acetaminophen, carbon tetrachloride, chloroform and methotrexate had no effect on mouse hepatocyte intercellular communication at non-cytotoxic levels. These results suggest that the ability to inhibit mouse hepatocyte intercellular communication is an effect specific to tumor promoters.Abbreviations DDT
1,1-Bis4-chlorophenyl]-2,2,2-trichloroethane
- FBS
fetal bovine serum
- LDH
lactate dehydrogenase
- TCA
trichloroacetic acid
- TPA
12-0-tetradecanoyl-phorbol-13-acetate |
| |
Keywords: | gap junction hepatocyte intercellular communication mouse tumor promoters |
本文献已被 SpringerLink 等数据库收录! |
|