Proline production via the arginine biosynthetic pathway: transfer of regulatory mutations of arginine biosynthesis into a proline-producing strain ofSerratia marcescens |
| |
Authors: | Masaki Sugiura Shin-ichi Suzuki Tsutomu Takagi Masahiko Kisumi |
| |
Institution: | (1) Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co. Ltd., Kashima, Yodogawa-ku, 532 Osaka, Japan |
| |
Abstract: | Summary Proline production via a part of the arginine biosynthetic pathway was examined. About 20 mg/ml ofl-proline was produced by using arginine biosynthetic enzymes. Accordingly, three mutations of arginine biosynthesis, namely, derepression of arginine biosynthetic enzymes (assigned byargR2), feedback inhibition-resistant N-acetylglutamate synthase (assigned byargA2) and defectiveness in N-acetylornithine aminotransferase (assigned byargD
–) were introduced by three transductional crosses into a proline-producing strain which produced about 55 mg/ml ofl-proline. The constructed strain produced 62 mg/ml ofl-proline, although about 10 mg/ml ofl-arginine and 1 mg/ml of N-acetylglutamate- -semialdehyde were produced as by-products. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|