An efficient protocol for genetic transformation of <Emphasis Type="Italic">Platycodon grandiflorum</Emphasis> with <Emphasis Type="Italic">Agrobacterium rhizogenes</Emphasis> |
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Authors: | Nam Il Park Pham Anh Tuan Xiaohua Li Yong Kyoung Kim Tae Jin Yang Sang Un Park |
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Institution: | (1) Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon, 305-764, Korea;(2) Department of Plant Science, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea;(3) Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea; |
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Abstract: | The balloon flower (Platycodon grandiflorum) is a popular traditional medicinal plant used in Korea to treat conditions such as bronchitis, asthma, tuberculosis, diabetes,
and inflammatory diseases. Recently, immunopharmacological research identified triterpenoid and saponin as important active
compounds in P. grandiflorum. To study and extract these compounds and other metabolites from P. grandiflorum, a technique was developed for producing hairy root cultures, which are a reliable source of plant compounds. To achieve this,
the activity of Agrobacterium rhizogenes was exploited, which can transfer DNA segments into plant genomes after infecting them. In this study, the A. rhizogenes strain R1000 was determined that had the highest infection frequency (87.5%) and induced the most hairy roots per plant,
and the concentration of antibiotics (75 mg/l kanamycin) was elucidated for selection after transformation. Wild-type and
transgenic hairy roots contained various phenolic compounds, although both of them had similar concentrations of phenolic
compounds. In the future, the protocols described here should be useful for studying and extracting valuable metabolites such
as phenolic compounds from P. grandiflorum hairy root cultures. |
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