Physicochemical properties of pretreated poplar feedstocks during simultaneous saccharification and fermentation |
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Authors: | Lisbeth Meunier-Goddik Michelle Bothwell Kunruedee Sangseethong Kuakoon Piyachomkwan Yun-Chin Chung Khamphet Thammasouk Djuhartini Tanjo Michael H Penner |
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Institution: | * Departments of Food Science and Technology, Oregon State University, Corvallis, OR 97331-6602, USA † Bioresource Engineering, Oregon State University, Corvallis, OR 97331-6602, USA |
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Abstract: | Physicochemical properties of native and dilute acid pretreated (0.6% H2SO4, 10 min, and either 170°C or 180°C) poplar were investigated before and during simultaneous saccharification and fermentation (SSF). SSF duration was 5 days and employed Trichoderma reesei cellulases and Saccharomyces cerevisiae fermentation. Chemical composition (glucan, xylan, lignin), enzyme-accessible surface area (based on solute exclusion), crystallinity index, particle size distribution, particle shape, and enzyme adsorption (cellulase, β-glucosidase) were compared to cellulose conversion. Cellulose conversion varied from 8% for native poplar to 78% for the 180°C-pretreated poplar. The physicochemical properties of native poplar changed little during SSF. In contrast, the physicochemical properties of the 180°C-pretreated feedstock changed markedly. Enzyme-accessible surface area and β-glucosidase adsorption increased by 83% and 65%, respectively, as cellulose was removed from the feedstock. Crystallinity index and particle size (large fraction) decreased by 65% and 93%, respectively. Cellulase adsorption per unit weight increased initially (+45%) followed by a slight decrease (−13%). The same trends were observed, although to a lesser extent, for 170°C-pretreated feedstock. |
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Keywords: | Fermentation simultaneous cellulase poplar pretreatment physicochemical properties |
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