| 苏云金芽孢杆菌菌株YBT1535转cry1C基因菌的构建 |
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| 引用本文： | 鲁松清,刘子铎,喻子牛.苏云金芽孢杆菌菌株YBT1535转cry1C基因菌的构建[J].生物工程学报,2000,16(5):587-590. |
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| 作者姓名： | 鲁松清 刘子铎 喻子牛 |
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| 作者单位： | 1. 华中农业大学畜牧兽医学院病毒室
2. 华中农业大学微生物科学技术系,农业微生物农业部重点实验室,武汉,430070 |
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| 基金项目： | 国家高技术研究发展与计划项目(101-03-01-01)及九五攻关项目(96-C01-02-01)资助。 |
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| 摘 要： | 利用电脉冲将ｃｒｙ１Ｃ基因转子苏云金孢杆菌野生菌株ＹＢＴ１５３５，筛选得到３个转化子。质粒电泳、ＰＣＲ扩增及Ｓｏｕｔｈｅｒｎ杂交结果均证明，基因ｃｒｙ１Ｃ已转入菌株ＹＢＴ１５３５。生物测定结果表明，３个转化子对甜菜夜蛾的毒力比出发菌ＹＢＴ１５３５均有显著的提高，转化子ＹＢＴ１５３５－１和ＹＢＴ１５３５－３对小菜蛾和棉铃虫的生物活性与出发菌ＹＢＴ１５３５相近，而转子化子ＹＢＴ１５３５－２则有一定幅度
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| 关 键 词： | 苏云金芽孢杆菌 电脉冲转化 cry1C基因 杀虫剂 |
| 文章编号： | 1000-3061-(2000)05-0587-04 |
| 修稿时间： | 2000-01-03 |
Transfer of cry1C Gene into Bacillus thuringiensis by Electroporation to Construct Strain with Broader Insecticidal Activity |
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| LU Song-Qing,LIU Zi-Duo,YU Zi-Niu.Transfer of cry1C Gene into Bacillus thuringiensis by Electroporation to Construct Strain with Broader Insecticidal Activity[J].Chinese Journal of Biotechnology,2000,16(5):587-590. |
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| Authors: | LU Song-Qing LIU Zi-Duo YU Zi-Niu |
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| Institution: | LU Song-Qing
,LIU Zi-Duo
,YU Zi-Niu
;(Department of Microbial Science and Technology, Huazhong Agricultural University, Key Laboratory of Agro-microbioiogy, Ministry of Agriculture, Wuhan 430070) |
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| Abstract: | Three Transformants were selected by transferring cry1C into Bacillus thuringiensis strain YBT1535. Plasmid profiles, PCR and Southern blot result all proved that cry1C had been transferred into strain YBT1535. Bioassay results showed that the transformants of strain YBT1535 displayed significantly higher toxicity against Spodoptera exigua than strain YBT1535, but the toxicities against Heliothis armigera and Plutella xylostella did not rise except transformant YBT1535-2. |
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| Keywords: | Bacillus thuringiensis gene cry1C electroporation |
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