A live cell hormone-binding assay on transgenic bacteria expressing a eukaryotic receptor protein |
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Authors: | Romanov Georgy A Spíchal Lukás Lomin Sergey N Strnad Miroslav Schmülling Thomas |
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Affiliation: | Free University of Berlin, Institute of Biology/Applied Genetics, Germany. gar@ippras.ru |
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Abstract: | The investigation of hormone-receptor interaction normally needs isolation and extensive purification of the receptor protein or a particular receptor-containing fraction. To bypass these time- and resource-consuming procedures, we have established a live cell-based assay using transgenic bacteria expressing single eukaryotic receptors. Here we describe some biochemical features of the Arabidopsis cytokinin receptor CRE1/AHK4 expressed in Escherichia coli. The data show that the main characteristics of the ligand-receptor interaction, including binding affinity and ligand specificity, can be determined using intact bacteria expressing a functional receptor. |
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Keywords: | Hormone Receptor Cytokinin Binding assay Transgenic Escherichia coli |
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