基于AFLP分子标记的桂花品种核心种质的构建

用100个桂花品种荧光AFLP分子标记信息构建核心种质。利用获得的指纹信息,运用UPGMA聚类取样法,采用Kimura 2-parameter遗传距离,多次聚类随机抽样。结果表明:(1)8对引物共获得514条带,平均每对引物获得64条带。(2)从100个桂花品种中筛选了30个样本的核心种质。(3)比较核心种质和全部种质的Shan-non-Wiener指数(H′)和Simpson指数(D),t检验值均说明核心种质的遗传多样性指数与全部种质遗传多样性没有明显差异,表明所构建的核心样品能够很好地保留原始100个桂花品种的遗传多样性。

Development of Core Collection of Osmanthus fragrans Lour.Cultivars Based on AFLP Molecular Markers

Amplified fragment length polymorphism(AFLP) markers were used to develop a core collection from 100 Osmanthus fragrans cultivars.Eight AFLP primer combinations produced a total of 514 polymorphic fragments with an average of 64 per primer combination.According to AFLP molecular markers of O.fragrans cultivars,an random sampling strategy was used to construct core collection using UPGMA cluster method according to Kimura 2-parameter genetic distance.30 samples were selected out of 100 O.fragrans cultivars as the core collection.Genetic diversity(Shannon-Wiener index and Simpson index) of the core collection was tested.The genetic diversity in the entire collection has been preserved in the core collection.