Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset |
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Authors: | Andrés Tittarelli Margarita Santiago Andrea Morales Lee A Meisel and Herman Silva |
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Institution: | (1) Millennium Nucleus in Plant Cell Biotechnology (MN-PCB), Santiago, Chile;(2) Plant Functional Genomics & Bioinformatics Lab, Universidad Andr?s Bello, Santiago, Chile;(3) Centro de Biotecnolog?a Vegetal, Universidad Andr?s Bello, Santiago, Chile |
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Abstract: | Background Cold acclimation is the process by which plants adapt to the low, non freezing temperatures that naturally occur during late
autumn or early winter. This process enables the plants to resist the freezing temperatures of winter. Temperatures similar
to those associated with cold acclimation are also used by the fruit industry to delay fruit ripening in peaches. However,
peaches that are subjected to long periods of cold storage may develop chilling injury symptoms (woolliness and internal breakdown).
In order to better understand the relationship between cold acclimation and chilling injury in peaches, we isolated and functionally
characterized cold-regulated promoters from cold-inducible genes identified by digitally analyzing a large EST dataset. |
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