Analysis of <Emphasis Type="Italic">Azospirillum brasilense</Emphasis> plasmid loci coding for (Lipo)polysaccharides synthesis enzymes |
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| Authors: | E I Katsy L P Petrova O V Kulibyakina A G Prilipov |
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| Institution: | 1.Institute of Biochemistry and Physiology of Plants and Microorganisms,Russian Academy of Sciencest,Saratov,Russia;2.Ivanovsky Institute of Virology,Russian Academy of Medical Sciences,Moscow,Russia |
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| Abstract: | Lipopolysaccharides LpsI and LpsII containing the same O-specific polysaccharide (OPS), yet different in antigenic structure
and charge, have been revealed in the rhizobacterium Azospirillum brasilense Sp245. In the present work, four putative glycosyltransferase genes were identified in a 14-kbp fragment of a 120-MDa plasmid
of Sp245, p120-lpsKM348X. Insertional mutagenesis of one of them, encoding the predicted ADP-heptose:LPS-heptosyltransferase, resulted in LpsI
loss. By means of DNA hybridizations and PCR with primers specific towards several sites of p120-lpsKM348X, it was demonstrated that homologous segments of 120-MDa plasmids of A. brasilense strains Sp245 and Sp107, which are characterized by identical structures of the OPS repeating units, are practically identically
organized. In an 85-MDa plasmid of Sp245, a locus was identified with high homology to the plasmid genes of glycosyltransferases
and conserved membrane-bound proteins from a wide range of soil bacteria. |
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