Preparation, characterization and anticoagulant activity in vitro of heparin-like 6-carboxylchitin derivative |
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Authors: | Yang Jianhong Cai Jun Wu Kang Li Dinglong Hu Ying Li Guoxiang Du Yumin |
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Institution: | Department of Environmental Engineering, School of Environmental and Safety Engineering, Changzhou University, Changzhou, China. yangshenyang3@163.com |
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Abstract: | A series of heparin-like 6-carboxylchitin derivatives with different N-acetyl group and sulfate group contents were prepared. Their structures were characterized by element analysis, FT-IR, (13)C NMR, and gel permeation chromatography. Their anticoagulant activity in vitro was investigated for human plasma with respect to activated partial thromboplastin time (APTT). The results showed all 6-carboxylchitin derivatives prolonged APTT within the scope of studied degree of sulfation (0.28-1.03) and Mws (4.3-13.7 kDa). Their anticoagulant activity strongly depended on their structures. 3,6-O-sulfated group promoted the anticoagulant activity. Only incorporation of N-sulfated group into deacetylated 6-carboxylchitin could not improve the anticoagulant activity. But, N-sulfated group and O-sulfated group had the synergistic action, and N-sulfated group could promote the anticoagulant activity for the N,O-sulfated chitin derivatives. In addition, acetyl group took a role in the anticoagulant activity, too. |
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