Evaluation of the radioimmunoassay (RIA) for detection of Ciguatoxin (CTX) in fish tissues |
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| Authors: | L H Kimura M A Abad Y Hokama |
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| Institution: | Department of Pathology, John A. Burns School of Medicine, University of Hawaii, Manoa, Honolulu, Hawaii 96822, U.S.A. |
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| Abstract: | The results of the radioimmunoassay (RIA) procedure for the direct determination of ciguatoxin (CTX) in fish tissues are presented. The RIA procedure detected CTX in 93% of fish tissues obtained from clinically documented ciguatera cases. The immunoglobulin nature of the reactive serum factor in sheep immunized with CTX was ascertained by DEAE-cellulose chromatography and by the ability to distinguish toxic from nontoxic fish tissues. Furthermore, a longitudinal study with commercial Seriola dumerili proved the RIA procedure to be effective in protecting the consumer from ciguatera poisoning by eliminating the potentially hazardous fishes from the markets. It is suggested that the RIA procedure has potential use in surveying areas for fishery development and is of value in keeping potentially toxic large fish off the market. |
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