Spatial and temporal alterations of phospholipids determined by mass spectrometry during mouse embryo implantation |
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Authors: | Kristin E Burnum Dale S Cornett Satu M Puolitaival Stephen B Milne David S Myers Susanne Tranguch H Alex Brown Sudhansu K Dey and Richard M Caprioli |
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Institution: | 2. Departments of Biochemistry, Chemistry, Nashville, TN 37232;4. Departments of Biochemistry, Pharmacology, Nashville, TN 37232;11. Departments of Biochemistry, Vanderbilt University Medical Center, Nashville, TN 37232;8. Division of Reproductive Sciences, Cincinnati Children''s Research Foundation, Cincinnati, OH 45229 |
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Abstract: | Molecular events involved in successful embryo implantation are not well understood. In this study, we used MALDI imaging mass spectrometry (IMS) technologies to characterize the spatial and temporal distribution of phospholipid species associated with mouse embryo implantation. Molecular images showing phospholipid distribution within implantation sites changed markedly between distinct cellular areas during days 4–8 of pregnancy. For example, by day 8, linoleate- and docosahexaenoate-containing phospholipids localized to regions destined to undergo cell death, whereas oleate-containing phospholipids localized to angiogenic regions. Arachidonate-containing phospholipids showed different segregation patterns depending on the lipid class, revealing a strong correlation of phosphatidylethanolamines and phosphatidylinositols with cytosolic phospholipase A2α and cyclooxygenase-2 during embryo implantation. LC-ESI-MS/MS was used to validate MALDI IMS phospholipid distribution patterns. Overall, molecular images revealed the dynamic complexity of lipid distributions in early pregnancy, signifying the importance of complex interplay of lipid molecules in uterine biology and implantation. |
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Keywords: | imaging mass spectrometry phosphatidylcholine sphingomyelins phosphatidylinositol phosphatidylserine phosphatidylglycerol phosphatidylethanolamine |
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