Generation of transgenic asulam-resistant potatoes to facilitate eradication of parasitic broomrapes (Orobanche spp.), with the sul gene as the selectable marker |
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Authors: | T Surov D Aviv R Aly D M Joel T Goldman-Guez J Gressel |
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Institution: | (1) Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel Fax: +972 8 934 4181, E-mail: lpgress2@wiccmail.weizmann.ac.il, IL;(2) Department of Weed Research, Newe Ya’ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel, IL |
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Abstract: | Root-attaching parasitic flowering broomrapes (Orobanche spp.) are major constraints to vegetable, legume and sunflower production around the Mediterranean and elsewhere, with banned
methyl-bromide fumigation or land abandonment of these affected crops as the major “solutions” to the problem. We report the
specific generation of transgenic asulam-resistant potatoes as a way to eradicate this pest. The target-site resistance in
the crop allows the herbicide to be translocated from treated leaves to the parasite via crop roots. This inhibitor of dihydropteroate
synthase lethally then prevents folic acid biosynthesis in the parasite. Additionally, we demonstrate that asulam can be used
directly in selecting resistant transformants, without the need for another selectable marker.
Received: 18 June 1997 / Accepted: 17 July 1997 |
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Keywords: | Asulam Herbicide resistance Orobanche Potato Selectable marker |
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