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Isolation of an agent affecting the development of an internal parasitoid
Authors:Phyllis G Hotchkin  Harry K Kaya
Abstract:The development of an internal braconid parasitoid, Glyptapanteles militaris, is adversely affected when its host, Pseudaletia unipuncta, is infected with the Hawaiian strain of granulosis virus. A plasma-derived agent, isolated from virus-infected hosts, was shown to elicit developmental aberrations in the parasitoid similar to those observed in virus-infected hosts. This agent was isolated by ammonium sulfate precipitation, gel filtration, and anion exchange chromatography, and its molecular weight, established by gel filtration and SDS-polyacrylamide gel electrophoresis, was determined to be about 64,000. The dilution end point of the agent and some stability properties were also established.
Keywords:host-parasitoid-baculovirus  plasma-derived agent  Pseudaletia unipuncta  Glyptapanteles militaris
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