基于环介导等温扩增技术的微流控芯片在病原菌检测方面的研究进展

环介导等温扩增(LAMP)技术是一种新兴的核酸恒温扩增技术,与微流控芯片技术相结合,可实现对病原菌的快速检测,具有特异性强、灵敏度高、操作简单等优点。本文根据不同终产物的检测方法对目前检测病原菌的相关微流控LAMP芯片进行了分类与介绍,并对技术的改进和存在的问题进行了分析,以期为后续的相关研究提供参考。

Advances in the detection of pathogenic bacteria by using loop-mediated isothermal amplification-based microfluidic chip

Loop-mediated isothermal amplification (LAMP) is an emerging nucleic acid amplification technique under isothermal condition, which can combined with microfluidic chip to rapidly detect the pathogenic bacteria. It is of higher specificity, higher sensitivity and simpler operation. This article classified and introduced the LAMP-based microfluidic chip for the detection of pathogenic bacteria, based on the analysis of different end-point detection methods. And also, we analyzed the improvement and existing problems of this technology, which can provide great reference value for the further studies and industry development.